Plasmid

Part:BBa_K3819021:Design

Designed by: Luo SongWen   Group: iGEM21_UESTC-China   (2021-09-28)


Balac plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized to improve expression in yeast and the restriction site optimized to connect a linker directly.


Source

1 GAP promoter (BBa_K3819009) 2 alpha-factor(BBa_K3819010) 3 Balac(BBa_K3819002) 4 His tag (6xHis-tag)

References