Plasmid
Part:BBa_K3819021:Design
Designed by: Luo SongWen Group: iGEM21_UESTC-China (2021-09-28)
Balac plasmid
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized to improve expression in yeast and the restriction site optimized to connect a linker directly.
Source
1 GAP promoter (BBa_K3819009) 2 alpha-factor(BBa_K3819010) 3 Balac(BBa_K3819002) 4 His tag (6xHis-tag)